CHAIN’s vector tools and strains are developed by the Minton Group at SBRC Nottingham. The group and relevant publications must be acknowledged in the user’s publications.
pMTL80000 vector series
A modular shuttle vector series for use in clostridia.
Palindromic restriction sites located in the vector sequence allow easy and quick modification of all modules. Thus, the addition of new modules that carry specific functions is not hindered by the need to create a new vector, but can simply be designed to slot into the existing module.
Click here to see the original pMTL80000 vector series kit and its developments available to you through CHAIN.
The availability of the pMTL80000 series has allowed the formulation of a simple ‘roadmap’ for gene system development in clostridial species.
The system revolves around the creation of a truncated pyrE mutant. Such mutants are both uracil auxotroph and resistant to 5-Fluoroorotic acid. The pyrE mutant forms the basis of all future manipulations using any mutagen. This is, because regardless of the mutagen employed, the pyrE mutation can be complemented concurrently.
Click here to see the pyrE mutant and other strains available to you through CHAIN.
ACE Vector Series
Allele Coupled Exchange (ACE) allows the creation of complementation and overexpression mutants in Clostridium spp of bacteria.
ACE vectors may be used to introduce a DNA fragment of up to 28 kb, a prerequisite for the engineering of strains to contain new functions and pathways.
ACE can be used with our bacterial strains and pMTL80000 vectors to generate unique strains of industrially and medically important bacteria with stable genetic changes on the chromosome.